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Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD

Received: 5 December 2017     Published: 6 December 2017
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Abstract

CLARITY (Clear Lipid–exchanged Acrylamide–hybridized Rigid Imaging/ Immunostaining/ in situ–hybridization–compatible Tissue hydrogel) is a powerful, innovative, whole brain-clearing technology, and it has been successfully combined with the immunofluorescence staining to achieve the 3D visualization of some proteins or cells in mm-thick brain tissue or even the intact brains. These 3D information help to gain deeper understanding on the pathologic mechanism of some neuronal diseases (for example Parkinson and Alzheimer). 14-3-3 zeta is a highly-expressed protein in Alzheimer’s disease (AD) brain, which was closely related with the formation of Tau aggregation and neurofibrillary tangles. However, little useful information has been available concerning the 3D architecture of 14-3-3 zeta in AD disease. In this paper, the transgenic AD mice were used and the 1mm-thick brain slices were passively clarified. Immunofluorescence staining results showed that different from the control group, 14-3-3 zeta was mainly present around the hippocampus in AD mice. Additionally, the morphology of 14-3-3 zeta protein was filamentous with different lengths. This result will be helpful for exploring the in vivo role of 14-3-3 zeta protein during the progression of AD.

Published in International Journal of Biomedical Science and Engineering (Volume 5, Issue 6)
DOI 10.11648/j.ijbse.20170506.12
Page(s) 68-71
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

AD, CLARITY, 14-3-Zeta Protein, Immunofluorescence

References
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Cite This Article
  • APA Style

    Honghong Song, Juan Feng, Jian Li, Yang Xia, Dezhong Yao. (2017). Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD. International Journal of Biomedical Science and Engineering, 5(6), 68-71. https://doi.org/10.11648/j.ijbse.20170506.12

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    ACS Style

    Honghong Song; Juan Feng; Jian Li; Yang Xia; Dezhong Yao. Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD. Int. J. Biomed. Sci. Eng. 2017, 5(6), 68-71. doi: 10.11648/j.ijbse.20170506.12

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    AMA Style

    Honghong Song, Juan Feng, Jian Li, Yang Xia, Dezhong Yao. Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD. Int J Biomed Sci Eng. 2017;5(6):68-71. doi: 10.11648/j.ijbse.20170506.12

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  • @article{10.11648/j.ijbse.20170506.12,
      author = {Honghong Song and Juan Feng and Jian Li and Yang Xia and Dezhong Yao},
      title = {Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD},
      journal = {International Journal of Biomedical Science and Engineering},
      volume = {5},
      number = {6},
      pages = {68-71},
      doi = {10.11648/j.ijbse.20170506.12},
      url = {https://doi.org/10.11648/j.ijbse.20170506.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20170506.12},
      abstract = {CLARITY (Clear Lipid–exchanged Acrylamide–hybridized Rigid Imaging/ Immunostaining/ in situ–hybridization–compatible Tissue hydrogel) is a powerful, innovative, whole brain-clearing technology, and it has been successfully combined with the immunofluorescence staining to achieve the 3D visualization of some proteins or cells in mm-thick brain tissue or even the intact brains. These 3D information help to gain deeper understanding on the pathologic mechanism of some neuronal diseases (for example Parkinson and Alzheimer). 14-3-3 zeta is a highly-expressed protein in Alzheimer’s disease (AD) brain, which was closely related with the formation of Tau aggregation and neurofibrillary tangles. However, little useful information has been available concerning the 3D architecture of 14-3-3 zeta in AD disease. In this paper, the transgenic AD mice were used and the 1mm-thick brain slices were passively clarified. Immunofluorescence staining results showed that different from the control group, 14-3-3 zeta was mainly present around the hippocampus in AD mice. Additionally, the morphology of 14-3-3 zeta protein was filamentous with different lengths. This result will be helpful for exploring the in vivo role of 14-3-3 zeta protein during the progression of AD.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD
    AU  - Honghong Song
    AU  - Juan Feng
    AU  - Jian Li
    AU  - Yang Xia
    AU  - Dezhong Yao
    Y1  - 2017/12/06
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    N1  - https://doi.org/10.11648/j.ijbse.20170506.12
    DO  - 10.11648/j.ijbse.20170506.12
    T2  - International Journal of Biomedical Science and Engineering
    JF  - International Journal of Biomedical Science and Engineering
    JO  - International Journal of Biomedical Science and Engineering
    SP  - 68
    EP  - 71
    PB  - Science Publishing Group
    SN  - 2376-7235
    UR  - https://doi.org/10.11648/j.ijbse.20170506.12
    AB  - CLARITY (Clear Lipid–exchanged Acrylamide–hybridized Rigid Imaging/ Immunostaining/ in situ–hybridization–compatible Tissue hydrogel) is a powerful, innovative, whole brain-clearing technology, and it has been successfully combined with the immunofluorescence staining to achieve the 3D visualization of some proteins or cells in mm-thick brain tissue or even the intact brains. These 3D information help to gain deeper understanding on the pathologic mechanism of some neuronal diseases (for example Parkinson and Alzheimer). 14-3-3 zeta is a highly-expressed protein in Alzheimer’s disease (AD) brain, which was closely related with the formation of Tau aggregation and neurofibrillary tangles. However, little useful information has been available concerning the 3D architecture of 14-3-3 zeta in AD disease. In this paper, the transgenic AD mice were used and the 1mm-thick brain slices were passively clarified. Immunofluorescence staining results showed that different from the control group, 14-3-3 zeta was mainly present around the hippocampus in AD mice. Additionally, the morphology of 14-3-3 zeta protein was filamentous with different lengths. This result will be helpful for exploring the in vivo role of 14-3-3 zeta protein during the progression of AD.
    VL  - 5
    IS  - 6
    ER  - 

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Author Information
  • College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China

  • College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China

  • College of Life Science, Beijing University of Chinese Medicine, Beijing, China

  • College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China

  • College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China

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