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The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge

Received: 20 June 2018     Published: 21 June 2018
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Abstract

The effect of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on the hydrolysis of sludge were investigated under the optimum condition of alkaline protease hydrolysis. The results showed that as the concentration of SHMP was increased, the content of Soluble Chemical Oxygen Demand (SCOD), protein, and polysaccharide in the sludge hydrolysate gradually raised. When the concentration of SHMP was 0.1g/g·tss, the contents of SCOD, protein, and polysaccharide increased by 17.16%, 50%, and 9.72% respectively. With the increase of MO concentration, SCOD decreased gradually. When the concentration of SHMP was 0.02g/g·tss, the SCOD decreased by 10.3%. An increase of 13% in the protein content was noted, when the concentration was 0.02g/g·tss. On the contrary, there was a slight reduction of the polysaccharide content i.e. 2.4%. The concentration of the polysaccharide was found to be the lowest at the concentration of 0.002g/g·tss. From the aforementioned results combined with three-dimensional fluorescence map of sludge EPS, it was found that SHMP promoted the hydrolysis of sludge by alkaline protease and accelerated the dissolution of intracellular organic matter. Herein, the MO inhibited alkaline protease hydrolysis of sludge, which hindered the dissolution of intracellular organics.

Published in International Journal of Environmental Protection and Policy (Volume 6, Issue 2)
DOI 10.11648/j.ijepp.20180602.14
Page(s) 42-49
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Alkaline Protease, Sodium Hexametaphosphate, Methyl Orange, Three-Dimensional Fluorescence Spectroscopy

References
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  • APA Style

    Cao Lu, Xue Fei, Liu Eryan, Li Dengxing, Asif Hussain, et al. (2018). The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge. International Journal of Environmental Protection and Policy, 6(2), 42-49. https://doi.org/10.11648/j.ijepp.20180602.14

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    ACS Style

    Cao Lu; Xue Fei; Liu Eryan; Li Dengxing; Asif Hussain, et al. The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge. Int. J. Environ. Prot. Policy 2018, 6(2), 42-49. doi: 10.11648/j.ijepp.20180602.14

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    AMA Style

    Cao Lu, Xue Fei, Liu Eryan, Li Dengxing, Asif Hussain, et al. The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge. Int J Environ Prot Policy. 2018;6(2):42-49. doi: 10.11648/j.ijepp.20180602.14

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  • @article{10.11648/j.ijepp.20180602.14,
      author = {Cao Lu and Xue Fei and Liu Eryan and Li Dengxing and Asif Hussain and Taimore Hussain},
      title = {The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge},
      journal = {International Journal of Environmental Protection and Policy},
      volume = {6},
      number = {2},
      pages = {42-49},
      doi = {10.11648/j.ijepp.20180602.14},
      url = {https://doi.org/10.11648/j.ijepp.20180602.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijepp.20180602.14},
      abstract = {The effect of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on the hydrolysis of sludge were investigated under the optimum condition of alkaline protease hydrolysis. The results showed that as the concentration of SHMP was increased, the content of Soluble Chemical Oxygen Demand (SCOD), protein, and polysaccharide in the sludge hydrolysate gradually raised. When the concentration of SHMP was 0.1g/g·tss, the contents of SCOD, protein, and polysaccharide increased by 17.16%, 50%, and 9.72% respectively. With the increase of MO concentration, SCOD decreased gradually. When the concentration of SHMP was 0.02g/g·tss, the SCOD decreased by 10.3%. An increase of 13% in the protein content was noted, when the concentration was 0.02g/g·tss. On the contrary, there was a slight reduction of the polysaccharide content i.e. 2.4%. The concentration of the polysaccharide was found to be the lowest at the concentration of 0.002g/g·tss. From the aforementioned results combined with three-dimensional fluorescence map of sludge EPS, it was found that SHMP promoted the hydrolysis of sludge by alkaline protease and accelerated the dissolution of intracellular organic matter. Herein, the MO inhibited alkaline protease hydrolysis of sludge, which hindered the dissolution of intracellular organics.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge
    AU  - Cao Lu
    AU  - Xue Fei
    AU  - Liu Eryan
    AU  - Li Dengxing
    AU  - Asif Hussain
    AU  - Taimore Hussain
    Y1  - 2018/06/21
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ijepp.20180602.14
    DO  - 10.11648/j.ijepp.20180602.14
    T2  - International Journal of Environmental Protection and Policy
    JF  - International Journal of Environmental Protection and Policy
    JO  - International Journal of Environmental Protection and Policy
    SP  - 42
    EP  - 49
    PB  - Science Publishing Group
    SN  - 2330-7536
    UR  - https://doi.org/10.11648/j.ijepp.20180602.14
    AB  - The effect of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on the hydrolysis of sludge were investigated under the optimum condition of alkaline protease hydrolysis. The results showed that as the concentration of SHMP was increased, the content of Soluble Chemical Oxygen Demand (SCOD), protein, and polysaccharide in the sludge hydrolysate gradually raised. When the concentration of SHMP was 0.1g/g·tss, the contents of SCOD, protein, and polysaccharide increased by 17.16%, 50%, and 9.72% respectively. With the increase of MO concentration, SCOD decreased gradually. When the concentration of SHMP was 0.02g/g·tss, the SCOD decreased by 10.3%. An increase of 13% in the protein content was noted, when the concentration was 0.02g/g·tss. On the contrary, there was a slight reduction of the polysaccharide content i.e. 2.4%. The concentration of the polysaccharide was found to be the lowest at the concentration of 0.002g/g·tss. From the aforementioned results combined with three-dimensional fluorescence map of sludge EPS, it was found that SHMP promoted the hydrolysis of sludge by alkaline protease and accelerated the dissolution of intracellular organic matter. Herein, the MO inhibited alkaline protease hydrolysis of sludge, which hindered the dissolution of intracellular organics.
    VL  - 6
    IS  - 2
    ER  - 

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Author Information
  • College of Environmental Science and Engineering, Donghua University, Shanghai, China

  • College of Environmental Science and Engineering, Donghua University, Shanghai, China

  • College of Environmental Science and Engineering, Donghua University, Shanghai, China

  • College of Environmental Science and Engineering, Donghua University, Shanghai, China

  • College of Environmental Science and Engineering, Donghua University, Shanghai, China

  • College of Environmental Science and Engineering, Donghua University, Shanghai, China

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